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Analysis of Enzyme Performance at pH 5 and pH 8

Enzyme performance at different pH levels

The Excel file Practical 9.xlsx contains a single sheet named Sheet1 with enzyme performance data at different pH levels (pH 5 and pH 8). The data includes the following columns for each pH level:
  • Enzyme: Name of the enzyme.
  • pH: The pH level at which the enzyme's performance was measured.
  • Km: The Michaelis constant, indicating the substrate concentration at which the reaction rate is half its maximum.
  • Kcat: The turnover number, representing the maximum number of chemical conversions of substrate molecules per second that a single catalytic site will execute for a given enzyme concentration under the given conditions.
  • Kcat/Km: A measure of enzyme efficiency.
The data covers various enzymes, including MnP111, E4OH, E44H, and others, providing a comparison of their performance at two different pH levels.

There is no significant difference between km at pH 5 and pH 8

The paired t-test conducted to examine the difference in Km values between pH 5 and pH 8 resulted in a P-value of 0.9569. This high P-value suggests that there is no statistically significant difference in Km values at the two pH levels, supporting the hypothesis.

Compare the Kcat of the enzymes at pH 5 and 8

The comparison of Kcat values at pH 5 and pH 8 for various enzymes was visualized using a bar chart. The chart showcases how Kcat values differ between the two pH levels for each enzyme, providing a clear visual representation of the enzymatic activity under different pH conditions.

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